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However, animal sources of enzymes typically suffer from variability in availability and in the health of the ani mals, especially regarding potentially lethal viruses, such as the virus re sponsible for mad cow disease, which can enter the food chain Plant sources of enzymes are less variable, but their availability is nonetheless seasonal Today, fungi and bacteria grown in fermentation tanks are the most common source of enzymes because they are inexpensive and always available Furthermore, recombinant DNA technology is being used to de sign highly ef cient fungi and bacteria The enzyme market is typically divided into two categories, bulk and ne Bulk enzymes, such as chymosin used in food preparation, proteases used in detergents, and bacteria used for bioremediation, represent 90 per cent of the enzyme market and are produced in batches of several hundred kilograms.

_____=_

i2k 1- -47f-E-d- [(47f) 1/3 k 2d 2 + - 3d- ] -3 3

Identify the classes, and then de ne the scenarios to describe how the objects from those classes work together De ne the scenarios and then use them to de ne the classes

(2.3.30)

The term with imaginary part in the denominator of (2.3.30) is known as radiative correction, which arises for the same reasoning as when scattering by Rayleigh spheres was discussed in 2, Section 8.2 of Volume 1.

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Let the medium be discretized into rectangular parallelepipeds Vs of sizes dx x d y x d z . We let dx = ad, d y = bd, d z = cd where a, band c are dimensionless quantities and their ratios denote the relative sizes of the three sides of the rectangular parallelepiped. The exclusion volume Vb for the dyadic Green's function in this case will be an infinitesimal parallelepiped with dimensions Ox = aO, Oy = M and Oz = cO and the L is as given in (2.1.63). Note that the ratios of the sides of the finite small rectangular parallelepiped Vs is the same as that of the infinitesimal rectangular parallelepiped Vb. Then

it is useful to write a low frequency approximation of Go(R). We note that for R =I- 0

.

2- - " E - eikR { 2 (1 - ,;kR) 2 - - - } G(R) = -k 2A (R) = - 41rk 2R3 k (-R I+RR)+ R2 (R I-3RR)

(2.3.31) When expanding G(R) of (2.3.31), it is important to see that there is a singular part of 0(1/(k 2R 3)) that is non-integrable over the origin. We also have to expand to the leading term in the imaginary part because that accounts for radiative correction. The Green's function G(R), on expansion will give 0(1/(k 2R 3)) + 0(1/ R) + iO(k). Thus in (2.3.31), we write exp(ikR) '::::' 1 + ikR - k 2R 2/2 - ik 3R 3/6. We have to include -ik 3R 3/6 because this gives a term of order iO(k) when multiplied with the second term inside the curly bracket of (2.3.31). Thus for R =I- 0 and kR 1

Bioremediation, a form of biotransformation, refers to the use of enzymes to degrade toxic and environmentally harmful compounds One of the rst commercial uses of bioremediation was the use of a genetically mod i ed bacterium used to digest oil slicks following a spill in a tanker accident Fine enzymes, such as urease, insulin, and DNA polymerase, are used in di agnostics, pharmaceuticals, and in genetic-engineering applications Fine en zymes, which are produced by the gram or kilogram, and command prices.

+ RR)(l + ikR)

(1 - ikR) 2= -k 2R 2 k3 R 3 } R2 (R 1- 3RR)(1 +ikR- -2- - i --)

8~ {~3 (R 2] + R R) + 4~k]}

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